Lyon survived a Cristiano Ronaldo-inspired comeback from Juventus to hang on and book a Champions League quarter-final against Manchester City.
Leading 1-0 from February’s first leg, the French side were handed a soft early penalty which Memphis Depay converted in style.
That left Juve needing to score three times to progress and Ronaldo got one back from the penalty spot.
He then thundered in his 130th Champions League goal but Lyon held on.
Juventus manager Maurizio Sarri may have guided his side to a ninth straight Serie A title but they were lethargic throughout and his position could be under threat.
Juve have not won the Champions League since 1996 and the wait goes on at least another year.